Androgen induces expression of the multidrug resistance protein gene MRP4 in prostate cancer cells

C. Cai; J. Omwancha; C. L. Hsieh; L. Shemshedini

doi:10.1038/sj.pcan.4500912

Androgen induces expression of the multidrug resistance protein gene MRP4 in prostate cancer cells

C. Cai
, J. Omwancha
, C. L. Hsieh
, L. Shemshedini

Biology

University of Toledo

Research output: Contribution to journal › Article › peer-review

Abstract

Multidrug resistance-associated proteins (MRPs) may mediate multidrug resistance in tumor cells. Using a gene array analysis, we have identified MRP4 as an androgen receptor (AR)-regulated gene. Dihydrotestosterone induced MRP4 expression in both androgen-dependent and -independent LNCaP cells, whereas there was little detectable expression in PC-3 or normal prostate epithelial cells. Disruption of MRP4 expression renders LNCaP cells more sensitive to the cytotoxic effects of methotrexate but not etoposide. Analysis of human tissues showed detectable MRP4 expression only in metastatic prostate cancer. These results suggest that AR induction of MRP4 mediates resistance of PC cells to nucleotide-based chemotherapeutic drugs.

Original language	English
Pages (from-to)	39-45
Number of pages	7
Journal	Prostate Cancer and Prostatic Diseases
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/sj.pcan.4500912
State	Published - Mar 2007

ASJC Scopus Subject Areas

Oncology
Urology
Cancer Research

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abstract = "Multidrug resistance-associated proteins (MRPs) may mediate multidrug resistance in tumor cells. Using a gene array analysis, we have identified MRP4 as an androgen receptor (AR)-regulated gene. Dihydrotestosterone induced MRP4 expression in both androgen-dependent and -independent LNCaP cells, whereas there was little detectable expression in PC-3 or normal prostate epithelial cells. Disruption of MRP4 expression renders LNCaP cells more sensitive to the cytotoxic effects of methotrexate but not etoposide. Analysis of human tissues showed detectable MRP4 expression only in metastatic prostate cancer. These results suggest that AR induction of MRP4 mediates resistance of PC cells to nucleotide-based chemotherapeutic drugs.",

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AU - Omwancha, J.

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AU - Shemshedini, L.

PY - 2007/3

Y1 - 2007/3

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AB - Multidrug resistance-associated proteins (MRPs) may mediate multidrug resistance in tumor cells. Using a gene array analysis, we have identified MRP4 as an androgen receptor (AR)-regulated gene. Dihydrotestosterone induced MRP4 expression in both androgen-dependent and -independent LNCaP cells, whereas there was little detectable expression in PC-3 or normal prostate epithelial cells. Disruption of MRP4 expression renders LNCaP cells more sensitive to the cytotoxic effects of methotrexate but not etoposide. Analysis of human tissues showed detectable MRP4 expression only in metastatic prostate cancer. These results suggest that AR induction of MRP4 mediates resistance of PC cells to nucleotide-based chemotherapeutic drugs.

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Androgen induces expression of the multidrug resistance protein gene MRP4 in prostate cancer cells

Abstract

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