Invasion-promoting extracellular matrix composition enhances photodynamic therapy response in 3D pancreatic cancer models

Gwendolyn M. Cramer; Hamid El-Hamidi; Jonathan P. Celli

doi:10.1117/12.2252983

Invasion-promoting extracellular matrix composition enhances photodynamic therapy response in 3D pancreatic cancer models

Gwendolyn M. Cramer
, Hamid El-Hamidi
, Jonathan P. Celli

Physics

University of Massachusetts Boston

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is characterized by extracellular matrix-rich stromal involvement, but it is not clear how ECM properties that affect invasiveness and chemotherapy response influence efficacy of photodynamic therapy (PDT). To disentangle the mechanical and biochemical effects of ECM composition, we measured the effects of various combinations of ECM proteins on growth behavior, invasive potential, and therapeutic response of multicellular 3D pancreatic tumor models. These spheroids were grown in attachment-free conditions before embedding in combinations of rheologically characterized collagen 1 and Matrigel combinations and treated with oxaliplatin chemotherapy and PDT. We find that cells invading from collagen-embedded tumor spheroids, the least rigid ECM substrate described here, displayed better response to PDT than to oxaliplatin chemotherapy. Overall, our results support that ECM-mediated invading PDAC populations remain responsive to PDT in conditions that induce chemoresistance.

Original language	English
Title of host publication	Optical Methods for Tumor Treatment and Detection
Subtitle of host publication	Mechanisms and Techniques in Photodynamic Therapy XXVI
Editors	David H. Kessel, Tayyaba Hasan
Publisher	SPIE
ISBN (Electronic)	9781510605350
DOIs	https://doi.org/10.1117/12.2252983
State	Published - 2017
Event	Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI - San Francisco, United States Duration: Jan 28 2017 → Jan 29 2017

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10047
ISSN (Print)	1605-7422

Conference

Conference	Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI
Country/Territory	United States
City	San Francisco
Period	1/28/17 → 1/29/17

ASJC Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Keywords

3D tumor models
extracellular matrix
Pancreatic cancer
photodynamic therapy
tumor microenvironment

Access to Document

10.1117/12.2252983

Cite this

Cramer, G. M., El-Hamidi, H., & Celli, J. P. (2017). Invasion-promoting extracellular matrix composition enhances photodynamic therapy response in 3D pancreatic cancer models. In D. H. Kessel, & T. Hasan (Eds.), Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI Article 100470O (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10047). SPIE. https://doi.org/10.1117/12.2252983

Invasion-promoting extracellular matrix composition enhances photodynamic therapy response in 3D pancreatic cancer models. / Cramer, Gwendolyn M.; El-Hamidi, Hamid; Celli, Jonathan P.
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI. ed. / David H. Kessel; Tayyaba Hasan. SPIE, 2017. 100470O (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10047).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Cramer, GM, El-Hamidi, H & Celli, JP 2017, Invasion-promoting extracellular matrix composition enhances photodynamic therapy response in 3D pancreatic cancer models. in DH Kessel & T Hasan (eds), Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI., 100470O, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10047, SPIE, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI, San Francisco, United States, 1/28/17. https://doi.org/10.1117/12.2252983

Cramer GM, El-Hamidi H, Celli JP. Invasion-promoting extracellular matrix composition enhances photodynamic therapy response in 3D pancreatic cancer models. In Kessel DH, Hasan T, editors, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI. SPIE. 2017. 100470O. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2252983

Cramer, Gwendolyn M. ; El-Hamidi, Hamid ; Celli, Jonathan P. / Invasion-promoting extracellular matrix composition enhances photodynamic therapy response in 3D pancreatic cancer models. Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI. editor / David H. Kessel ; Tayyaba Hasan. SPIE, 2017. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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title = "Invasion-promoting extracellular matrix composition enhances photodynamic therapy response in 3D pancreatic cancer models",

abstract = "Pancreatic ductal adenocarcinoma (PDAC) is characterized by extracellular matrix-rich stromal involvement, but it is not clear how ECM properties that affect invasiveness and chemotherapy response influence efficacy of photodynamic therapy (PDT). To disentangle the mechanical and biochemical effects of ECM composition, we measured the effects of various combinations of ECM proteins on growth behavior, invasive potential, and therapeutic response of multicellular 3D pancreatic tumor models. These spheroids were grown in attachment-free conditions before embedding in combinations of rheologically characterized collagen 1 and Matrigel combinations and treated with oxaliplatin chemotherapy and PDT. We find that cells invading from collagen-embedded tumor spheroids, the least rigid ECM substrate described here, displayed better response to PDT than to oxaliplatin chemotherapy. Overall, our results support that ECM-mediated invading PDAC populations remain responsive to PDT in conditions that induce chemoresistance.",

keywords = "3D tumor models, extracellular matrix, Pancreatic cancer, photodynamic therapy, tumor microenvironment",

author = "Cramer, \{Gwendolyn M.\} and Hamid El-Hamidi and Celli, \{Jonathan P.\}",

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AU - Cramer, Gwendolyn M.

AU - El-Hamidi, Hamid

AU - Celli, Jonathan P.

PY - 2017

Y1 - 2017

N2 - Pancreatic ductal adenocarcinoma (PDAC) is characterized by extracellular matrix-rich stromal involvement, but it is not clear how ECM properties that affect invasiveness and chemotherapy response influence efficacy of photodynamic therapy (PDT). To disentangle the mechanical and biochemical effects of ECM composition, we measured the effects of various combinations of ECM proteins on growth behavior, invasive potential, and therapeutic response of multicellular 3D pancreatic tumor models. These spheroids were grown in attachment-free conditions before embedding in combinations of rheologically characterized collagen 1 and Matrigel combinations and treated with oxaliplatin chemotherapy and PDT. We find that cells invading from collagen-embedded tumor spheroids, the least rigid ECM substrate described here, displayed better response to PDT than to oxaliplatin chemotherapy. Overall, our results support that ECM-mediated invading PDAC populations remain responsive to PDT in conditions that induce chemoresistance.

AB - Pancreatic ductal adenocarcinoma (PDAC) is characterized by extracellular matrix-rich stromal involvement, but it is not clear how ECM properties that affect invasiveness and chemotherapy response influence efficacy of photodynamic therapy (PDT). To disentangle the mechanical and biochemical effects of ECM composition, we measured the effects of various combinations of ECM proteins on growth behavior, invasive potential, and therapeutic response of multicellular 3D pancreatic tumor models. These spheroids were grown in attachment-free conditions before embedding in combinations of rheologically characterized collagen 1 and Matrigel combinations and treated with oxaliplatin chemotherapy and PDT. We find that cells invading from collagen-embedded tumor spheroids, the least rigid ECM substrate described here, displayed better response to PDT than to oxaliplatin chemotherapy. Overall, our results support that ECM-mediated invading PDAC populations remain responsive to PDT in conditions that induce chemoresistance.

KW - 3D tumor models

KW - extracellular matrix

KW - Pancreatic cancer

KW - photodynamic therapy

KW - tumor microenvironment

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Invasion-promoting extracellular matrix composition enhances photodynamic therapy response in 3D pancreatic cancer models

Abstract

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ASJC Scopus Subject Areas

Keywords

Access to Document

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