Abstract
Significance: In in-line digital holographic microscopy (DHM), twin-image artifacts pose a significant challenge, and reduction or complete elimination is essential for object reconstruction. Aim: To facilitate object reconstruction using a single hologram, significantly reduce inaccuracies, and avoid iterative processing, a digital holographic reconstruction algorithm called phase-support constraint on phase-only function (PCOF) is presented. Approach: In-line DHM simulations and tabletop experiments employing the sliding-window approach are used to compute the arithmetic mean and variance of the phase values in the reconstructed image. A support constraint mask, through variance thresholding, effectively enabled twin-image artifacts. Results: Quantitative evaluations using metrics such as mean squared error, peak signal-to-noise ratio, and mean structural similarity index show PCOF s superior capability in eliminating twin-image artifacts and achieving high-fidelity reconstructions compared with conventional methods such as angular spectrum and iterative phase retrieval methods. Conclusions: PCOF stands as a promising approach to in-line digital holographic reconstruction, offering a robust solution to mitigate twin-image artifacts and enhance the fidelity of reconstructed objects.
| Original language | English |
|---|---|
| Article number | 076502 |
| Journal | Journal of Biomedical Optics |
| Volume | 29 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 1 2024 |
ASJC Scopus Subject Areas
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering
Keywords
- complex wave filtering
- lensless in-line digital holographic microscopy
- phase reconstruction
- phase-only function
- phase-support constraint
- twin-image elimination
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